Cell infection with lentivirus: GFP+ after soring by flow but no protein expression?

I use a plasmid to overexpress 1 protein in 293T and murine BMSC. But I could never succeed. I produced the lentivirus by transfecting the plasmids in HEK293FT cells, together with another 3for...

24 December 2020 6,389 3 View

I keep getting an error for GEOPIV on Matlab?

When doing the geoPIV_RG_run, i keep encountering this error. Error using dataread Trouble reading floating point number from file (row 1, field 2) ==> Dynamic Load Test Free Vibration Error...

20 October 2020 10,004 2 View

Is lentiviral production of antibodies in 293T viable?

Hi, I recently started using lentiviral systems to produce proteins in mammalian cells. Knowing the performance of this impressive system in producing high titers of proteins, I am wondering if...

05 August 2020 5,144 2 View

How to choose collective variables for ligand binding to protein?

Hello! What is the recommended way to choose collective variables / reaction coordinates to study ligand binding to a protein (e.g., for PMF free energy profiling)? I often see the advice like "Do...

10 March 2020 1,878 4 View

No title

02 November 2019 8,943 3 View

Why an SD of means difference between two groups (e.g. treatment vs. placebo) is equal to a particular SD in each group?

Very frequently in papers, devoted to parallel clinical trials, I face a situation, when a calculated SD for an effect in each group is approximately equal to an SD of effect difference between...

07 July 2019 9,217 4 View

How to find the collaborators for the joint project in the field of MRI/CT?

We launched a project on the recognition of haematoma in the CT of the human brain. This project was initiated on the basis of the Polenov Russian Research Institute of Neurosurgery. At the...

01 June 2018 1,293 3 View

What aproach we must use to calculate force in electrostatic problems: potential or electric field intensity?

Well known problem of finding potential for metal sphere and point charge, solving with "mirror image" trick give you 2 different answers for the force, acting on point charge. One when we use...

14 December 2016 9,365 5 View

Does choline hydroxide (46% aqueous) ionic liquid still absorb water from surroundings?

I will be using this ionic liquid as an electrolyte for my battery experiments.

01 August 2016 2,559 1 View

Hall Sensor and EMC?

What would be your brief recommendations for EMC for Hall sensors? I mean; how to increase robustness of the Hall sensors circuits? Any links? Thank you, Eugene

27 April 2016 8,388 9 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Which programs are to be used to register 2D image onto a 3D image data set?

I am trying to register a light micrograph onto a 3D CT data set, as I want to extract the corresponding image from the CT data set. Is there any software or program can do this accurately? I am...

21 February 2021 8,220 3 View

What is the better software for approximation of 3D surface with equations?

I have a massive of x, y, z coordinates of 2D surface in 3D space, which builded in Ansys HFSS or AutoCAD. How can I get the analitical aproximation of this surface by formulas?

21 February 2021 3,444 7 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View

How can I obtain Reaction Forces in ABAQUS?

I am trying to simulate a simplified version of a beam resting on 10 points using Abaqus. The solutions I want to obtain are the Reaction Forces in each resting point or area when applying a...

21 February 2021 3,075 2 View

How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

20 February 2021 6,967 1 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Has anyone used deep learning for 2D/3D REGISTRATION ?

I am a Master student in Biomedical (Bio-electrical) Engineering and work on " 2D-3D registration ( CT and X-Ray) as my thesis. I created DRR from CT and want use CNN( Convolutional Neural...

19 February 2021 6,354 3 View

PV system design. 3d modelling and Analysis?

I have been looking for a good cost-effective tool for 3D modelling a glass-glass solar module system. I was initially considering Skelion as a tool but it does not look very promising, a bit...

13 February 2021 8,126 1 View