What software can be used to add Hydrogen to 3D structure of small RNA hairpin?

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Why does everyone use vs code?

Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...

07 August 2024 7,013 4 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

I have no added any resarch paper yet but showing three paper? how to delete it?

I have not addede any paper yet but it has selected 3 papers which are not mine in my account. i want to delete that information. please help me

30 July 2024 3,743 0 View

How much sample we are required to do a pilot study for standardized an Achievement Test?

I am conducting a study on Effectiveness of e-content on academic achievement of secondary school science student.

17 July 2024 5,633 4 View

Does anyone know, any conference related to "mathematical data compression" topic?

Please suggest me, I want to present my research work.

13 July 2024 1,461 1 View

?como elimino articulos que no son propios?

eliminoar articulos que son de tu autoria

05 July 2024 2,107 1 View

Any professor of Social Entrepreneurship ?

PhD in Social Entrepreneurship and Digital Technologies

04 July 2024 1,675 2 View

What is the maximum acceptable degradation of gain in the orthogonal plane relative to the beam scanning plane for beam steering antennas?

In research papers on beam steering antennas, while the radiation pattern for the beam scanning plane (such as the horizontal or vertical plane) is typically presented, details regarding the...

03 July 2024 8,321 0 View

How can I synthesize beads of silica?

Hi to all of you! My goal is to create synthetic silica beads. These beads ought to have a specified size. Could someone please advise on how to proceed or give me the articles I need to finish my...

02 July 2024 8,170 1 View

Can we use MTCMOS instead of CMOS for designing SRAM Latch ?

Basically CMOS are used for designing the SRAM cells . How the functionality will differ if we use Multi threshold CMOS instead of CMOS generally MTcmos decrease the leakage power but it will...

28 June 2024 9,845 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Gromacs first step of minimization ?

I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...

07 August 2024 2,598 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View