The concentrations of DNA extracted from avian stool samples are extremely low ( about 0.5 ng/ uL, using nanodrop spectrophotometer). May someone suggest me any suggestion to improve my extraction methods using Qiamp Fast DNA Stool Mini Kit for running the NGS to identify the diet analysis using ANML primer set. I have done exactly as the manual provided by the kit but still got low DNA concentration which the highest that I can is about 6.7 ng/uL but A260/A280 is 1.05 and A260/A230 is 0.21.
thank you.
Use Qubit if possible for concentration measurement. To increase concentration of extracted DNA, 1) use more input sample, or 2) you can use less elution buffer at the last elution step, and 3) longer incubation before the final centrifugation step (5 min instead of 1) can also increase the DNA yield
Péter Gyarmati I strongly recommend using the PCI (Phenol-chloroform-isoamyl alcohol) method as it will give you more DNA than any other method and I strongly recommended using ethanol (70%) for washing.
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