I used Trizol Reagent to isolate RNA. I did what the protocol says. Furthermore, I used 10 ug Glycogen during the RNA precipitation step because of a small number of cells. After ethanol washing, I dissolved the RNA pellet in RNase free water however it did not dissolve. I put my samples into a heat blocker at 58 C. What sould I do?
Trizol obtained RNA pellet should NOT dry under speedvac because if you over-dry it you have to put it in dustbin, no way to dissolve. I had the same problem and after that I dry it at 37 deg for 5-10 min and after it dissolves very easily. Also what Matthias said is correct too but I had the same issue with starch rich tissues.
Trizol obtained RNA pellet should NOT dry under speedvac because if you over-dry it you have to put it in dustbin, no way to dissolve. I had the same problem and after that I dry it at 37 deg for 5-10 min and after it dissolves very easily. Also what Matthias said is correct too but I had the same issue with starch rich tissues.
I know that your problem does not require any further comments as it's been a while..., but next time you may try to precipiate your RNA sample again (the bit of dissolved RNA will precipitate too which results in no loss of RNA). Thereby you may decrease your salt concentration, making your pellet more easily dissolvable. Also, it gives you another opportunity not to overdry the pellet.
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