9 Questions 17 Answers 0 Followers
Questions related from Emre Can Tüysüz
KRAS,NRAS,BRAF wild type colorectal cancer can response the anti-EGFR therapy using cetuximab or panitumumab. However, in some cases such as Her2 or MET amplification there will be an resistant to...
01 January 2015 8,527 10 View
I used Trizol Reagent to isolate RNA. I did what the protocol says. Furthermore, I used 10 ug Glycogen during the RNA precipitation step because of a small number of cells. After ethanol washing,...
10 October 2014 7,843 4 View
See above.
09 September 2014 7,853 8 View
I sort cells and culture it but when I culture it CSC population reduces. I sort CD133+CD15+ double positive cancer cells. I check purity of cancer stem cells and it is always more than %90. I...
09 September 2014 3,155 8 View
I isolate total RNA from approximately 10k-15k cell through Trizol reagent. However, the purity of RNA sample is very low. Can you recommend a kit for total RNA isolation from small quantities sample?
04 April 2014 1,782 8 View
I used an X-treme siRNA transfection reagent to transfect miRNA. After that I performed Real-Time PCR to validate my transfection. I do my transfection at 2 different times (8 hour and 72 hour)....
12 December 2013 2,391 3 View
In my experiment, I will use a one type of human Glioblastoma cell line for a positive control. I have U-87 Gbm cell line but U-87 cell line has astrocytoma disease, too. Can I use it? Or which...
11 November 2013 973 16 View
I have prepared cell culture from glioblastoma tissue however I should show that this cell culture is a glioblastoma cell culture. How I can prove it? What is the best marker gene that is...
11 November 2013 1,786 2 View
I performed real time PCR for miRNA. I used SYBR Green Master Mix for miRNA and 5S primers for control. For my samples I should get a peak at 20 cycles. There is no problem in this step. However,...
08 August 2013 2,316 3 View
