Thomas Gantert

3 Questions 30 Answers 0 Followers

Questions related from Thomas Gantert

Vanadate and EDTA in cell lysis buffer, conflicting?

Dear fellow scientists, I stumbled upon literature stating that the commonly used phosphotyrosyl phosphatase inhibitor sodium orthovanadate is potently chelated by EDTA. This may be a problem when...

04 April 2016 1,218 4 View

Do you know of a 'biomarker' of the activity of protein synthesis (translation)?

I observed markedly different weights of a specific organ in my animal model upon treatment. My hypothesis is that this change is largely driven by protein. I would like to examine if the...

01 January 2015 1,242 8 View

What is the protocol for rendering HepG2 cells insulin resistant?

Is there anyone who can provide protocols for the induction of insulin resistance in HepG2 through palimitate, TNF-alpha and LPS ?

03 March 2014 3,853 4 View