What should I do for my controls not to stain my secondary antibody ( IHC for mice muscle) ?

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Jason Rogers

First question to get the obvious one out of the way, is your secondary a Mouse Ab?

Also you're in STL, go ask Rodney in IHC at BJH if he's still there (used to work overnights,) he'll set you up if you ask nice.

Margaret Nalugo

No, secondary was produced in Donkey serum ( its a donkey anti rabbit). Yes I am in STL, Whose lab? which floor?

Main AP Histology lab. Used to be 3rd floor in the Main hospital.