What should be the parameters or tests needed to perform to establish the anticancer effects of a drug against DMBA induced breast cancer in mice?

More Yahyea Baktiar Laskar's questions See All

What will be the impact of ongoing wars and bombardments in global warming and pollution?

In the current wars in Ukraine vs Russia, and Gaza-palestine vs Israel , large-scale bombardments, missile attacks, grenades, firearms and ammunitions are used, leading to production of tremendous...

08 February 2024 5,529 0 View

How much loading dye should I add with the sample so that the protein sample will properly work in SDS PAGE?

I have prepared 12% of SDS gel and a sample concentration that needs to load 24 ul in each lane to fulfill the requirement of 4uM protein concentration and the maximum volume of the lane is 20 ul....

06 June 2023 4,734 1 View

How to solve the fatal error while using Gromacs?

I am using Gromacs MD simulation for protein-ligand complex using the following command after NVT Equilibration : gmx grompp -f npt.mdp -c nvt.gro -t nvt.cpt -r nvt.gro -p topol.top -n index.ndx...

02 November 2022 6,754 2 View

What are the tools available for computational predication of chemical synthesis of a particular compound?

I'm trying to predict the retrosynthesis of an experimental compound from its SMILE string and 3D structure using IBM RXN Chemistry Server. I got some results but not sure about their reliability....

03 May 2022 7,445 3 View

What is applicability domain in QSAR studies?

What is the applicability domain in QSAR studies and how to estimate that? I tool (VLifeMDS) I'm using for QSAR model generation also provides applicability domain analysis. However, this giving...

07 June 2021 9,296 3 View

How to prepare .lib and .frcmod parameter files for ligands in AMBER forcefield?

I'm trying to perform a molecular dynamics simulation of a protein-ligand complex. For that, I need to prepared ligand parameter files in .lib and .frcmod format. Is there any online server that...

07 May 2021 2,404 2 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 2,251 4 View

Can we consider a molecule as a "drug lead" if it has a positive charge in its structure?

Can we consider a positively charged molecule as a drug lead while designing a drug using computational tools?

07 September 2020 9,453 3 View

What is the best sensing technique for fever detection of COVID-19 and other viral fever patients?

Please propose best sensing technique for fever detection of COVID-19 and other viral fever patients

21 April 2020 2,980 2 View

Will anybody give his account or narration with respect to problems on application of sensors in industry?

19 November 2019 3,806 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View