What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?
Mr. Laskar,
You should use mass spectrometry (MS,) instead of, only a HPLC analysis. Because of, only the former method is capable of quantifying analytes absolutely, selectively, sensitively, precisely and accurately, respectively.
Furthermore, there should be used our own-authored (mine and my co-author's contribution according to the authorship shown, below) innovative stochastic dynamic method and model formulas for exact determination of MS experimental measurable variables 'intensity' of peaks of analytes. It has been tested, so far, at mixtures of analytes at pg.(mL)-1 an dng.(L)-1 concentration levels, showing linear correlation coefficients between theory and experiment /r/ = 0.99997-1.
Please, consider detail on references [1,2].
[1] Stochastic Dynamic Mass Spectrometric Approach to Quantify Reserpine in Solution; Bojidarka Ivanova, Michael Spiteller
Analytical Chemistry Letters, 10 (2020) 703-721; Received 13 Oct 2020, Accepted 16 Dec 2020, Published online: 28 Jan 2021
Download citation https://doi.org/10.1080/22297928.2020.1865834
[2] Steroids, 164 (2020) 108750
Stochastic dynamic mass spectrometric quantification of steroids in mixture — Part II; Bojidarka Ivanova, Michael Spiteller
Perhaps, it would be useful to you to consider, as well as, reference [3]. It deals, with mass spectrometric analyses of ingredients of hop plant. Some of these compounds have structurally very similar to anthocyanins molecular fragments.
[3] Bojidarka Ivanova and Michael Spiteller
Chapter 4. Quantitative Analytical Methods to Organic Ingredients in the Quality Control Process for Beer Production; In: Beer: Production, Consumption and Health Effects; W. Salazar (Ed.), (2016) pp. 1 - 296;NOVA Science Publishers, New York, ISBN: 978-1-63485-704-8.
The following papers maybe useful.
doi: 10.3390/molecules22030478
doi: 10.1002/bmc.3275
doi: 10.1016/j.jchromb.2006.08.002
doi: 10.1002/jssc.200700149
salam.the following freshl published would helpp you,good luck.
Food Research International
Volume 138, Part A, December 2020, 109754.
Validated liquid chromatography separation methods for identification and quantification of anthocyanins in fruit and vegetables: A systematic review
Author links open overlay panelMamathaChandra SinghabCelineKelsobcWilliam E.PricecYasmineProbstab
Analysis of anthocyanins in commercial fruit juices by using nano-liquid chromatography-electrospray-mass spectrometry and high-performance liquid chromatography with UV-vis detector
Chiara Fanali 1, Laura Dugo, Giovanni D'Orazio, Melania Lirangi, Marina Dachà, Paola Dugo, Luigi Mondello .
Using Mass spectrometry which useful method for structural analysis with in valuable assistance from unclear magnetic resonance spectroscopy
- Badges
- Science method