What should be the maximum length of a primer?

10 October 2018 0 8K Report

Recently I designed a primer that is 50 bp long with 9 bp overhang.GC% is 32 and Tm 63C. I made this long primer just to get good GC%. Do you guys think I made any mistake? On this note, my forward primer has just 36 base pairs(GC% 44 and T m 65C ).

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