I lysed my intertidal phototrophic biofilm with hypotonic lysing solution containing 10 Mm Tris, pH 8.0, 1 Mm EDTA, 0.5% Triton-X 100, 1x protease inhibitor cocktail. after complete homogenization and centrifugation at 10000 rpm for 12 minutes at 4 degree Celsius the resulting supernatant is green in color. Is this the right color of the supernatant indication proper extraction of total protein or the color should be bright blue?

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