Hi,

I need to incubate isolated human PMBC for a longer duration (10-15 days). Hence, I will wash them with PBS and change media every 3 days. How should I set up centrifuge parameters to prevent cell loss and preserve viability to make cell pellets from PBMCs? I tried 200g for 10 minutes at room temperature but that left more cells in the supernatant than precipitate.

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