I'm going to design a study to test whether an untested protein could be a biomarker for pacreatic cancer. I intend to do immunohistochemistry of the pancreatectomy tissue of the patients with pancreatic cancer, benign tumour and disease - free. I will also do Western blotting and ELISA. Could you please suggest me in terms of positive control selection for these tests?
Hi Saif! I'm not too familiar with Westerns and ELISA but I have done extensive IHC work on muscle and skeletal tissue in mouse cancer models. Typically you'd check the data sheet on any given Ab to see what positive controls the manufacturer recommends. Given that your Ab is untested, I would simultaneously stain for an Ab which will be widely expressed in any tumor alongside your untested Ab. I would recommend CD68, a marker of the myeloid lineage and macrophages. Also, CD31, a marker of endothelial cells is expressed robustly in tumors. Hope this helps!
http://www.abcam.com/cd68-antibody-kp1-ab955.html
http://www.abcam.com/cd31-antibody-ab28364.html
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