Dear all,
I want to do a NGS of my sample that happens to be rumen digesta. Now I am confused as regards what should be my sample, the solid digesta or the liquid squeezed out from that solid part? I read some papers wherein they have squeezed out liquid from the solid digesta, centrifuged at 15000g at 15min and then extracted DNA from the pellet.
So what should I do?
Dear Yasir,
Depending on the type of sample, its quality, and the objectives of your work you will have to decide the method.
In general terms, the best option is to follow the most common technique used in your field and published in the Journal of higher impact, so that you can cite them and justify your methodology.
Hello brother Yasir..
I recommend you to use and follow this protocol (PowerLyzer PowerSoil DNA Isolation Kit..)
i used it before .. let me know if you have question
thanks,
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