Yasir Bashir

6 Questions 9 Answers 0 Followers

Questions related from Yasir Bashir

How to obtain genomic DNA fragments for library construction?

Hi all! I am trying to construct a genomic DNA library of a gram-positive bacterium. I have isolated the genomic DNA and now I have to chop off the DNA into small fragments for cloning into a...

15 April 2023 4,009 5 View

How to calculate Alpha and beta diversity of a shotgun metagenomic data?

Hi everyone! I hope you all are fine. I have tried many tutorials regarding the calculation of alpha and beta diversity, however, my RStudio is throwing up so many issues. Packages aren't getting...

17 January 2023 2,287 3 View

How to calculate the abundance of Antibiotic Resistance Genes in a shotgun metagenomics data?

Hi, I got the shotgun sequencing data of my samples and I ran RGI for my samples to check the ARGs present in my samples using CARD database. Now I want to calculate the abundance of ARGs. May I...

18 October 2022 5,959 2 View

Anyone working on Metagenomic DNA analysis?

Any body working on metagenomic DNA analysis in my connections? I need some help as regards the said analysis.

08 January 2022 5,478 3 View

How does a gene of 3.2 kb size get ligated into a plasmid of 2.7 kb size practically?

I have constructed a DNA library and I have got an insert of around 3.2 kb size showing desired phenotype. How does a gene this big in size, get ligated to a vector much lesser in size than it?

28 May 2016 7,973 4 View

What should be my sample for Next generation sequencing of rumen digesta? The solid part or the liquid part??

Dear all, I want to do a NGS of my sample that happens to be rumen digesta. Now I am confused as regards what should be my sample, the solid digesta or the liquid squeezed out from that solid...

09 February 2016 9,063 3 View