Assuming that the PCR condition is optimal, but I still get primer dimers that I have to get rid of for downstream experiments, what ratio should I use to keep my fragments as much as possible?
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Liutao Chen
I think you can follow the manufacture instrcution to get the right size of fragment and concentrate the beads by removing the supernatant to get more you wanted fragment
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Liutao Chen
As i done ,the 0.6 Xbeads can absorb > 500 bps fragment ,0.8Xbeads can absorb > 300 bp beads,you may have a try, by the way ,I used beckman company beads for my experiments and reconmend you do the same.
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Katie A Burnette
You shouldn't have any problem with your 3 kb product. Just follow the manufacture instructions and run some out of a gel to make sure your dimers are gone.
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