Lu Yang

6 Questions 2 Answers 0 Followers

Questions related from Lu Yang

What ratio of AMPure beads/sample should I use to remove short primer dimers but keep as many my 3kb fragments as possible?

Assuming that the PCR condition is optimal, but I still get primer dimers that I have to get rid of for downstream experiments, what ratio should I use to keep my fragments as much as...

08 August 2017 8,299 4 View

How often can I get an indel using crispr NHEJ?

If the gRNA works, chances are there is a small indel at the cutting site, but how frequent will this happen? Can I use the length difference as an indicator of gRNA efficiency.

09 September 2016 9,659 1 View

What is the reliable Ct value range in qPCR?

I'm doing qPCR on four copies of ATPase alpha. The expression level varies a lot and the Ct values at two cDNA concentrations are as below: cDNA Copy 1 Copy 2 ...

01 January 2016 3,642 4 View

What's the difference between STAR 2-pass mapping with or without re-generated genome?

I'm working on an uncommon insect. There's a reference genome for it but the quality is not that good. I decided to use STAR 2-pass to get my data better mapped, but I'm not sure if I should...

11 November 2015 9,464 2 View

For a reference for RNAseq analysis, should I merge my own transcriptome assemblies or use a published genome from a near species?

I want to do a RNAseq analysis of Papilio.glaucus and now we have the sequences from 12 samples. I need a reference if I want to use Cufflinks/Tophat2. Should I merge my own transcriptome...

08 August 2015 2,956 6 View

Will the 3' polyA tail be washed away if I purify PCR product with beads?

I want to clone a 3kb fragment. I use Phusion first to increase the fragment, run a gel, cut the size I want, purify the gel, use Taq to add 3' poly A tail, purify the PCR product, and then do the...

07 July 2015 9,352 5 View