'm doing SNP genotyping in the chickpea association panel, I have fetched 1KB up and downstream sequences flanking SNPs. m trying to find out restriction sites at the particular position i.e. the restriction sites within SNPs. Please provide the details except for the NEB cutter.
Geneious Prime has a feature to annotate restriction sites if you have access to it (they offer a free trial)
Check https://royalsocietypublishing.org/doi/10.1098/rsos.172054
https://soar-ir.repo.nii.ac.jp/record/3987/files/SNP_detection.pdf
Check https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-13-77
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0124781&type=printable
https://medlineplus.gov/genetics/understanding/genomicresearch/snp/
Kindly look at this paper.
https://iopscience.iop.org/article/10.1088/1755-1315/888/1/012024/pdf
- Badges
- Science method