Cre-loxp system does not work (lines #017455 and #017320, JAX). Why?

We bought this year two lines (#017455 and #017320) to get specific expression of channelrhodopsin2 (ChETA, in this case) in parvalbumin-positive (PV) interneurons. We hoped that crossing a mouse...

16 November 2020 8,701 1 View

How could I determine the starting methyltransferase enzyme and methyl donor to methylate a synthetic peptide?

Hello, I have a synthetic peptide with arginine residue I aim to methylate using PRMT1 + SAM (methyl donor). I then will use my dimethylated arginine peptide product in ELISAs and Western blots....

12 November 2020 5,568 7 View

ELISA-Why does my diluted high absorbance sample have such lower concentration compared to less diluted sample when using standard curve?

Hello! Our lab has developed a standard curve with known concentration that we use to determine our ELISA concentrations. We then multiply by the dilution factor for our final concentration....

25 October 2020 8,601 6 View

What is the difference between Social Science Citation Index (SSCI) and Emerging Science Citation Index (ESCI)?

31 May 2020 5,819 4 View

How to handle the analysis of yield stability or yield risk based on simulated crop yield data?

Dear everyone, do you have any experience on the analysis of yield stability or yield risk based on simulated crop yield data? I would like to use a modelling approach and simulate grain yields...

23 February 2020 1,584 6 View

What is the best way to calculate the yield of an entire crop rotation including different kind of crops ?

What is the best way to calculate the yield of an entire crop rotation including different kind of crops and different yield levels ? For example the following cropping sequences: 1. sugar beet...

03 December 2019 9,527 11 View

Protocol for whole heart tissue lysis for Westerns?

Hello, I am troubleshooting a western protocol using whole heart homogenates but I seem to be getting consistent smearing at the lower band range of my gel which is also where I want to see my...

01 October 2019 8,682 4 View

What is the minimum sample size for factor analysis?

I will be doing ryff's psychological well-being scale for psychometric properties. What would be the minimum sample size for factor analysis using spss? I read Slovin's formula is not reliable?

26 April 2017 601 7 View

Could alternate form reliability be administered simultaneously?

I will be doing ryff's psychological well being scale's psychometric properties. Could I administer alternate form reliability simultaneously? (42-item and 18-item)

25 April 2017 8,184 3 View

IS Confrimatory Factor Analysis CFA and WLS enough for psychometric evaluation?

Im going to do a psychometric evaluation on ryff's scale using nurses as my sample. Would CFA and WLS suffice? Thank you!

13 April 2017 4,938 3 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

What are the harmful effects of these extreme rainfall on the health of newborns? What are the reasons for that?

Extreme rainfall associated with climate change is causing harm to babies in some of the most forgotten places.

01 March 2021 5,647 4 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View