We bought this year two lines (#017455 and #017320) to get specific expression of channelrhodopsin2 (ChETA, in this case) in parvalbumin-positive (PV) interneurons. We hoped that crossing a mouse with cre-recombinase in PV-interneurons (#017320) with a mouse which has genes of a marker and channelrhodopsin-2 between poxp-sites we get desirable animal. But the system doesnot work by some reason. We have genotyped both lines, we have crossed several paires, using female #017320 and male #017455 and vice versa. The G1 generation did not have any fluorescent neurons in any brain region and neurons did not response to 470nm or 505nm light, in particular PV-interneuons. If you have any idea how to make system working, please, help. Thank you.
Dear Max,
thank you very much for the advice. I have checked the entorhinal cortex, CA1 and DG of the hippocampus in the ages 3 weeks-2 months.
Bedt Regards,
Elena
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