Is evolution steered by gene expression?

06 July 2017 9,218 4 View

How do I improve the stability of gold nanoparticle films?

04 May 2015 2,937 7 View

Can anyone recommend a carboxylate silane?

04 May 2015 454 10 View

Couly you recommend an anti-BSA antibody vendor?

03 April 2015 855 2 View

How do I compute the reflectance from nanoparticle films?

We've successfully computed the reflectance from a multilayered system of glass, organosilanes and gold nanoparticle films (SEE ATTACHED FIGURE) using the transfer matrix method, and treating the...

03 April 2015 5,990 4 View

How can I stir very small volumes (down to 200uL) with stirrer?

We need to stir a 200-300uL volume in a microtube, and currently are relying on flea stirrers; however, even the smallest stirrer we can find always needs cut with bolt cutters to get a small...

03 April 2015 9,038 6 View

Does a 1:1 binding model require that 100% sites are filled?

In regard to label-free assays developed to measured interactions between some target molecule and a surface, I often see the Langmuir isotherm/1:1 binding model applied to systems without...

03 April 2015 7,991 5 View

How to include papers in a thesis beyond "stapling"?

02 March 2015 2,786 1 View

How to use pictures from a published article?

02 March 2015 6,829 4 View

Can I cite an article twice?

02 March 2015 4,843 35 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View