I have isolate iron oxidizing bacteria using K2HPO4 (0.4 g / L), MgSO4.7H2O (0.4 g / L), (NH4) 2SO4 (0.4 g / L) and FeSO4.7H2O (33.3 g / L) and gellan gum as a solidifying agent, pH 3.0-3.5 on petriplates. Growth of bacteria takes place 5-7 days but in broth bacteria growth was not seen. I am troubling in getting my bacterial strain identified as it is not being amplified. so can you plz suggest any solution in this regard?

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