I am working on lignolytic bacterial enzymes for ethanol production. During pretreatment of the biomass, many inhibitors are thought to be produced. So I want to analyze my samples for different inhibitors. Please suggest if you are aware of it. Thanks
Dear Sir. Concerning your issue about determination of inhibitors in hydrolysate sample using spectrophotometer. A set of in vitro assay conditions were selected for the determination of ACE-inhibitory activity, and the need was demonstrated to standardize this assay so that the results obtained by different authors may be comparable. The conditions selected were as follows: 10 mM HHL concentration in 0.2 M potassium phosphate buffer and 0.3 M NaCl and 26 mU of ACE/mL as reaction medium; incubation time, 80 min at 37 °C. The method was applied to the study of ACE-inhibitory activity of dairy product and wine samples. Of the samples assayed, it was infant formulae whey that produces the greatest ACE inhibition. Red wine also presents a high inhibition percentage. This latter sample has an important matrix effect that must be corrected in the calculation. ACE-inhibition type was also studied, using a yogurt whey and a Captropil solution as substrates. The whey produced noncompetitive inhibition and the Captropil competitive inhibition. I think the following below links may help you in your analysis:
http://www.scielo.br/pdf/babt/v57n3/cd_1738.pdf
http://pubs.acs.org/doi/abs/10.1021/jf034148o
https://www.ncbi.nlm.nih.gov/pubmed/23993489
Thanks
Dear Friend,
I think my answer going to help you in some point of view. For estimating the different inhibitors present in the hydrolysate, you need to analyze using HPLC is the right way and the reviewer will not ask you any questions.If you go for spectroscopic methods, then the reviewer willnot accept that method for publication. May be it will be used for your knowledge. SO i request you to characterize using HPLC/GC depends on the molecules.
Dear Islam Eldin Hussein and Govindarajan,
Thank you so much for your valuable suggestions and kind help.
Thanks...
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