What percentage of sheep blood is the best for making BHI broth medium for fecal bacterial culture?

10 October 2013 1 2K Report

I want to make BHI broth and add blood to it. Is it okay if I add blood to the broth media without filtration or I need to filter it?

Rachel L Robson

? you can't filter the blood, of course, as that will remove all the RBCs, which I assume you want. And, assuming it's from a healthy animal, the blood is already sterile/free of bacteria.

BHI is so thick it doesn't filter especially well on its own.

Bottom line: Autoclave the BHI broth to sterilize it (this is recommended anyway), cool it to room temperature, and then add the blood using sterile pipets and aseptic technique. I'd make a big flask of BHI, then add the blood, then aliquot into single-use tubes.

5% v/v blood should be good for most things.

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