Mohsen Sohrabi

14 Questions 24 Answers 0 Followers

Questions related from Mohsen Sohrabi

Which parameter are suitable for my analysis? Paired, unpaired, parametric tests or noparametric tests?

XCMS will be used for comparison of E. coli metabolites and Salmonella metabolites. The method of analysis is LCMS analysis of overnight cultured media. First dataset is replicate of E. coli...

07 July 2014 2,231 1 View

What is the best DNA extraction method from mix bacterial culture in broth media?

I have 10 ml LB inoculated by mix bacteria (G+ and G-). It may include several different phyla, genera and species. I am going to extract their DNA. I am looking for any good protocol or kit.

07 July 2014 6,164 2 View

What is the difference between PCA-none scaling and PCA-Unit variance-center?

I am going to compare two groups of bacteria based on their metabolites. PCA; Principle Component Analysis

06 June 2014 2,981 1 View

Is it possible to analyze volatile organic compounds by HPLC/MS or ESI-LCMS)?

I am going to analyse VOCs in bacterial culture media.

04 April 2014 2,921 5 View

What are the differences between peptone, tryptone, tryptone peptone, trypticase, and trypticase peptone?

Reference to "Caldwell, D.R. and M.P. Bryant, Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria. Applied microbiology, 1966. 14(5): p. 794-801" I need to use...

03 March 2014 4,131 12 View

How to make M10 culture medium?

Can you help me with the best way of making M10 bacterial culture medium for anaerobic bacteria.

02 February 2014 1,735 2 View

How can I compare 40 GC/MS chromatograms to find VOCs profile?

I run the same GC/MS condition for 40 different fecal samples to analyze VOCs. Now, I have 40 chromatograms and I want to find the profile of VOCs (common in more than 80% of all fecal samples).

11 November 2013 5,026 5 View

Which one will give me better resolution in microbiota diversity analysis; Sanger or Pyrosequencing?

For intestinal microbiota analysis which culture independent method (Sanger and Pyrosequencing) can analyse bacterial identification at the level of species.

11 November 2013 5,723 2 View

How I can find the similarities of several GC/MS chromatograms? Can I relay of retention time or the best one is retention index?

I cultured a bacterium in 8 different culture conditions to find the VOCs cohort of this bacterium in different conditions. I am wondering if you can tell me the best way of data analysis by using...

10 October 2013 8,655 4 View

What is the best method for bacterial characterization?

Bacterial characterization.

10 October 2013 3,614 21 View

What percentage of sheep blood is the best for making BHI broth medium for fecal bacterial culture?

I want to make BHI broth and add blood to it. Is it okay if I add blood to the broth media without filtration or I need to filter it?

10 October 2013 1,565 1 View

Which kits or method can be used for DNA extraction from mixture of unknown bacteria in bacterial culture?

I need to extract DNA of bacterial culture. The problem is that culture media is not pure and it contains mixture of bacteria. Most of the kits that I have found is for DNA extraction from pure...

07 July 2013 9,237 9 View

What is the best method for bacterial identification at the level of species?

For pure culture and also mix culture.

07 July 2013 7,864 2 View

Which level of identification can be acheived by using 454 pyrosequencing for gut microbiome?

Level of bacterial identification by 454 pyrosequencer for 16S rRNA sequencing.

07 July 2013 2,874 4 View