What is the best DNA extraction method from mix bacterial culture in broth media?

I have 10 ml LB inoculated by mix bacteria (G+ and G-). It may include several different phyla, genera and species. I am going to extract their DNA. I am looking for any good protocol or kit.

06 July 2014 6,040 2 View

Which parameter are suitable for my analysis? Paired, unpaired, parametric tests or noparametric tests?

XCMS will be used for comparison of E. coli metabolites and Salmonella metabolites. The method of analysis is LCMS analysis of overnight cultured media. First dataset is replicate of E. coli...

06 July 2014 2,132 1 View

What is the difference between PCA-none scaling and PCA-Unit variance-center?

I am going to compare two groups of bacteria based on their metabolites. PCA; Principle Component Analysis

05 June 2014 2,900 1 View

Is it possible to analyze volatile organic compounds by HPLC/MS or ESI-LCMS)?

I am going to analyse VOCs in bacterial culture media.

03 April 2014 2,719 5 View

What are the differences between peptone, tryptone, tryptone peptone, trypticase, and trypticase peptone?

Reference to "Caldwell, D.R. and M.P. Bryant, Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria. Applied microbiology, 1966. 14(5): p. 794-801" I need to use...

02 March 2014 3,381 12 View

Which one will give me better resolution in microbiota diversity analysis; Sanger or Pyrosequencing?

For intestinal microbiota analysis which culture independent method (Sanger and Pyrosequencing) can analyse bacterial identification at the level of species.

10 November 2013 5,642 2 View

How can I compare 40 GC/MS chromatograms to find VOCs profile?

I run the same GC/MS condition for 40 different fecal samples to analyze VOCs. Now, I have 40 chromatograms and I want to find the profile of VOCs (common in more than 80% of all fecal samples).

10 November 2013 4,926 5 View

How I can find the similarities of several GC/MS chromatograms? Can I relay of retention time or the best one is retention index?

I cultured a bacterium in 8 different culture conditions to find the VOCs cohort of this bacterium in different conditions. I am wondering if you can tell me the best way of data analysis by using...

09 October 2013 8,525 4 View

What is the best method for bacterial characterization?

Bacterial characterization.

09 October 2013 3,503 21 View

What percentage of sheep blood is the best for making BHI broth medium for fecal bacterial culture?

I want to make BHI broth and add blood to it. Is it okay if I add blood to the broth media without filtration or I need to filter it?

09 October 2013 1,445 1 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View