I am preparing liposomes to mimic E.coli membrane to study functional parameters of membrane proteins. Along with using PE and PG, I am interested in understanding how lipid A interacts with membrane proteins and therefore, I need to mimic the membrane which is physiologically relevant with respect to its lipid A profile. Can anyone tell me what is the mol percentage of lipid A found in the composition of E.coli plasma membrane.
Maybe here do you could info about your question .
http://www.binasss.sa.cr/revistas/rccm/v16n3/art8.pdf
http://bacmap.wishartlab.com/organisms/791
The outer membrane bilayer consists of an inner leaflet composed of ordinary phospholipids and an outer leaflet composed of lipopolysaccharide, of which lipid A is the membrane part. There are also proteins embedded in the outer membrane. The main problem with simulating the outer membrane lipid with liposomes is the asymmetry: How do you make the inner and outer leaflets out of different lipids?
Here is a paper in which asymmetric lipid bilayers were made:
http://rsif.royalsocietypublishing.org/content/10/89/20130810
Thank you very much. It's really helpful. I actually wanted to know the lipid A content in the plasma membrane not the outer membrane. If there is anyone who might have separated the two membranes and doe lipid content analysis of plasma membrane?
Lipid A is found in the outer membrane. The cytoplasmic membrane consists of ordinary phospholipids. However, there is probably a little bit of lipid A in the inner membrane at any given time because it is made there and then transported to the outer membrane by the lipopolysaccharide transport system (LptABCDE).
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