What percentage of acridine orange solution should be used in fluorescent microscopy?

More Anurag Satpathy's questions See All

Which MWCO of Slide-A-Lyzer™ MINI Dialysis Devices is required to remove Low molecular weight contaminant removal like ethanol from LNPs ?

Hello everyone, I have made Lipid nano particles through microfluidics but unable to remove organic solvent from it, recently i came across to know about Dialysis Devices used to remove Low...

06 March 2024 634 2 View

Is there a reluctance among the Indian retailers in adopting AI technologies to their Supply Chain Practices?

AI is the new transformative technology. Many countries like China, US and Germany are investing a lot in the technology and adopting it into their industries. But India seems to be lagging behind...

22 January 2024 6,711 0 View

What can be the source of debris in brain slice IHC stainings and and how to get rid of it?

I am staining for VGLUT2 in mouse brain sections. I am seeing a lot of debris on the slices and these are visible in both Green and Red channels. By looking at the sizes I am sure that these are...

17 January 2024 1,340 0 View

Please answer this 1. If we are typing any scientific name in the forat "Genus spp." than what should be in italic ?

2. If we are simply typing the genus of any scientific name (like Rhizoctonia) then it should be in italics or not? Please answer these. Thank You.

28 October 2023 9,263 4 View

How to Plot radiation pattern in origin pro?

I currently working on microstrip patch anntena, i completed my design on CST software. Know i need to plot the radiation pattern of co polar and cross polar of E&H plane separately on origin...

17 October 2023 548 1 View

How water seepage will affect the stability of drive in metal mining?

Sometimes water from exploratory boreholes or water accumulated in old working seeps in through the roof of the drive, in long run can this seepage weaken the rock strength?

06 October 2023 5,921 2 View

Is there any tool for measurement of relaxation in psychometric domain?

Relaxation response is an event facilitated by several practices and lifestyle. I am working on such a practice.

25 September 2023 7,115 3 View

What are the side effects of using compressed air for ventilating blind headings along with duct?

Sometimes near the face air is not able to reach properly and if the heading length is more then 100m then sometimes as a easy solution compressed air which is used to run machines is used to...

23 September 2023 5,812 0 View

How we can define 'General Knowledge'?

Often, we come across this term 'General Knowledge' (GK) at various platforms. People generally use this term without any care and put everything under the preview of GK. As I think, ‘GK is any...

15 September 2023 1,843 1 View

How can we make Homeopathic dilution of any plant extracts?

What is the basis of making of such dilutions? Is the common method of preparing the dilutions used for all plant extract? Or does it depend on the plant extract and its reported biological...

19 June 2023 3,297 0 View

Is the use of soap and water enough (acceptable) to eliminate or breakdown acridine orange/ethidium bromide dual dye (ao/eb)?

I have an experiment involving acridine orange/ethidium bromide dual dye (AO/EB). How can I safely eliminate or inactivate these toxic chemicals afterward? and how to clean up the lab in case of...

08 January 2024 8,052 3 View

Why lots of nucleus appears red in fish erythrocytes when i am conduct micronucleus assay with acridine orange?

Hi everyone, i use acridine orange for micronucleus assau but lots of nucleus is red. Is anyone have this problem, can someone help me why all the cells red? Thanks so much for your time already.

24 October 2023 9,496 2 View

How can I calculate the pH of internal organelles using a ratiometric pH method?

How can I calculate the pH of internal organelles using a ratiometric pH method? Which formulas should I use after acquiring the fluorescent measurements? I have employed Lysosensor Blue-Yellow...

16 August 2023 4,753 1 View

What may be the reason behind the degeneration/degradation of 96h zebrafish larvae during the AO and DCFH-DA staining to study the apoptosis and ROS?

20-25 larvae (96h) were randomly selected, washed 2-3 times with PBS, and then stained with AO(5ug/ml) and DCFH-DA(10uM) stain for 30 min separately and then washed with PBS 2-3 times to remove...

27 July 2023 9,672 4 View

How can I interpret the fluorescent readings taken using a 96-well plate and a Plate reader with a dual staining Acridine Orange and Propidium Iodide?

As you are aware, Acridine orange is an intercalating dye that can permeate both live and dead cells. AO will stain all nucleated cells to generate green fluorescence. Propidium iodide can only...

13 June 2023 7,613 9 View

Acridine orange staining on cells resulting from an enzymatic digestion?

I want to know if it is possible to make acridine orange staining (to detect apoptosis) on cells resulting from enzymatic digestion of tissue by dispase and preserved with acetic acid methanol. Thanks

09 June 2023 2,663 2 View

What is the best way of assessing viability in a NK92 cell culture?

The cells form clumps which I understand is a good sign. I would like to work with a reliable and accurate live cell count, but every counting method has given very variable results, so it's hard...

19 January 2023 8,603 2 View

Acridine orange and propidium iodide (AOPI) titration for flow cytometry of peripheral blood mononuclear cells (PBMCs)?

Hi guys, I am looking to optimize viability staining on peripheral blood mononuclear cells (PBMCs) with acridine orange and propidium iodide (AOPI). I will be plotting a titration curve of...

11 April 2022 6,197 0 View

Does agent orange cause skin lesions?

My husband has been battling the VA for years for skin lesions we believe are caused by exposure to agent orange. Does anyone have any research specific about it? - Patricia McAfee.

28 February 2022 6,266 2 View

Looking for a fluorescent mitochondria marker that is not affected by membrane potential or ROS production?

Hi, I'm searching for a fluorescent mitochondria marker (to be used in live confocal microscopy) that is not dependant of membrane potential nor ROS production. I'll have MitoSox to quantify ROS...

09 February 2022 4,312 3 View

Jordan R. Yaron

5 µg/mL for 10 minutes at 37C, then wash three times according to Life Technologies.

Anurag Satpathy

Thank you very much for your input Yaron JR.

Rahul Pratap Singh

Concentarion of acridine orange 6 mg/ml is sufficient to fluorescent microscopy, Acridine Orange solution is stable for several weeks when stored at 4°C and in the dark.

Noémie Hamilton

I used 3µg/ml for zebrafish embryos for 15min at 27C, then 3 washes of 10 minutes each. Whether you are imaging cells or embryos, imaging has to be fast as photoactivation of AO by UV light will trigger some amazing explosions of the lysosomes! Nice if this is what you want to see, but not so nice when it happens before the imaging is done...