Hi, Fredrick!
To identify this species conidiophore structure is necessary besides a colony view. And it would be helpful to use Czapek agar and Malt agar for Penicillia as a range of manuals have descriptions for these media. It is important because Penicillium morphology is exteremely variable and can depend on media.
Good luck,
Elena
Hi, Fredrick!
To identify this species conidiophore structure is necessary besides a colony view. And it would be helpful to use Czapek agar and Malt agar for Penicillia as a range of manuals have descriptions for these media. It is important because Penicillium morphology is exteremely variable and can depend on media.
Good luck,
Elena
As far as colony color and morphology of fungus is concerned I also agree with Elena. However you should perform PCR amplification of its ITS region and DNA sequencing of PCR amplified product. You can also check Penicillium vanoranjei (Orange penicillium), which has been recently described in 2013.
Beautiful colonies of no doubt Penicillium. But Microscopic pics are necessary.
It can be quite a gamble to identify a fungal species based on an image of its cultural appearance. However, if you can observe biverticillate symmetrical conidiophores like the one shown in the attached picture, then it most likely is a species belonging to the Penicillium subgenus Biverticillium, currently ascribed to the genus Talaromyces
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It is Penicillium, also need to growth on Czapek agar and Malt agar to see shape of the growth with pigmentation, then must do microscope to see a shape the conidiophore and the branch of conidiophore, with metula
Thank you
Hi, Fredrick. Congratulations. You've got a relatively pure culture of saprotrophic fungi. Unfortunately, only.
For identification you are should to see the conidiophores. You can grow of fungi exclusively on selective medium, to visually identify where, again under directories. In this case, it will be easier to look for a cluster on Genbank.
Another approach - to hold a simple analysis of the known DNA markers (for example on internal transcriber spacer or other specific zones in DNA or fragment on high specific for your Penicillium genes). This method will show exactly which group of fungi colonies are grown up on your plate.
For example, I can offer to see my article.
But this steps also can not help, then have to call the professionals.
Penicilles are very different and diverse. Good luck.
In this site you are can see the different species of Penicillium https://en.wikipedia.org/wiki/List_of_Penicillium_species
It looks like a member of Penicillium section Sclerotiora.
Colony morphology on CYA and MEA media, microscopic characteristics and sequences of ITS, BenA and CaM are necessary to identify it correctly.
For definitive identification of Penicillium species, it is necessary to use molecular methods such as sequencing of beta tubulin gene.
Yes, Mahdi Abastabar . The molecular method on tubulin gene is correct and high informatively.
Hi Fedrick
It is difficult to exactly explain the species looking at the colony character.It would be appreciable if you could load a microphotograph of the same LCB staining. But it may be marnefeei as it is showing orange pigmentation.
its like to be as talaromyces genus but need to check special characteristics on media such as YES and CYA and shape of conidiophore ,Conidiophores with biverticillate phialides was most probably belong to talaromyces genus
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