19 October 2023 2 5K Report

Hi. I am relatively new to sequencing workflows and am wondering what are some standard packages, tools, and practices from processing Sanger sequencing data? For more context - we have forward and reverse reads of about 500-650bp length from various unknown specimens. We would like to take the raw reads (.ab1), trim and filter them, and then create consensus reads to be written to a fasta file. The fasta files will be uploaded to NCBI blastn for reference comparison. Any insight would be appreciated. Thank you.

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