I'm planning to use the MAB377 anti-NeuN (a very common antibody) for staining of primary hippocampal neurons in culture. The vendor website recommends diluting 1:10 - 1:100 for ICC and 1:100 - 1:1000 for IF, and all the references I checked only refer to using the antibody for IF purposes. I would have expected the concentrations used for ICC and IF to be reversed, so I don't know if I trust this! Based on your experience, is 1:10-1:100 a legitimate range for ICC? If not, what dilutions are you all using?

Thank you for your insight!

Vendor website:

https://www.emdmillipore.com/US/en/product/Anti-NeuN-Antibody-clone-A60,MM_NF-MAB377#anchor_REF