Jessica Anderson

2 Questions 1 Answers 0 Followers

Questions related from Jessica Anderson

What NeuN staining concentration to use for primary cell culture?

I'm planning to use the MAB377 anti-NeuN (a very common antibody) for staining of primary hippocampal neurons in culture. The vendor website recommends diluting 1:10 - 1:100 for ICC and 1:100 -...

12 August 2021 8,139 1 View

Is media with phenol red acceptable when performing ICC/IF or smFISH on primary neuronal cultures?

Hi all, thank you for your lending time and your experience to my question! I understand phenol red is discouraged when pursuing live imaging because it interferes with the fluorescence....

12 July 2021 7,842 1 View