To anyone who happened to grow this strain,
I am using Nutrient Broth to grow it in both liquid and agar. However, after 5 days, I cannot notice a visible concentrating of the cells.
Hi ,
You can use YPD (yeast extract (1%), peptone (2%), dextrose (2%)) medium and may take 24 / 48hrs to grow.
Metal-containing heterogeneous catalysts often need to be calcined before reduction, so what are some examples of catalysts that perform better without calcination? What is the reason and how many...
05 August 2024 909 2 View
Prof. Chiu and I are looking for a post-doctoral fellow who is interested in working on assessment/feedback research (see the link at the end of this post for more details). If your friends or...
16 July 2024 5,549 6 View
I have noticed that, regardless of whether isolating primary microglia from humans or mice, purity is generally assessed using CD11c flow cytometry staining. Why not use CD11b instead?
02 July 2024 9,411 2 View
What are the recent applications of chitosan in the field of environmental photocatalysis? Have you ever implemented chitosan based environmental photocatalysts
06 May 2024 3,737 0 View
Hi everyone, I have a question that has been bothering me for a month. In Abaqus Explicit, I used shell (S4R) to simulate a compression experiment. I chose 'general contact' with 'all* with...
22 April 2024 3,975 4 View
Hi all, I want to have 301 copies/ul for my downstream experiments. The sample I am using is a purified plasmid, of which I know the concentration (ng/ul), volume (ul), and molarity(g/mol). How...
20 April 2024 2,468 4 View
I would like to expand memory B cells from a single memory B cell, and we are working on establishing hCD40L expressing -3T3 or -293T cell lines. Before we get the hCD40L-cell lines, we have tried...
25 March 2024 5,077 0 View
I need to calculate the free energies of simple cations such as Cs+ and anions like Br- & I- in a water-DMF (1:3) mixed solvent, where some of these ions have complex interactions with...
22 March 2024 1,629 0 View
Have you encountered any major barriers to cross-cultural communication about language differences, any non-verbal cues, or the way preconceptions and stereotypes come into play? If so, how did...
07 March 2024 448 1 View
Should we prohibit the false statements from the papers published in academia journals? The words that “Einstein's famous equation E=mc2” is a sentence directly copied from internet. It is a...
04 February 2024 5,499 15 View
Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.
09 August 2024 5,605 2 View
I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...
08 August 2024 4,812 2 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
How is matter moved through an ecosystem and movement of energy different from the movement of nutrients through ecosystems?
03 August 2024 4,859 0 View
Why does the flow of energy through the biosphere depend on the cycling of nutrients and matter and energy flow across the earth's subsystem?
03 August 2024 6,412 0 View
How is the flow of matter through an ecosystem different from the flow of energy matter and nutrients flow through an ecosystem?
03 August 2024 8,655 0 View
I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....
31 July 2024 3,843 7 View
How far is the distance with question marks on it? There is a river, where you measure at the point out to the right and there is a low screen. The river is green, what is visible is within the...
24 July 2024 4,405 1 View
One has to list down the details and understand that different feedstocks and the biogas digesters working on different feedstocks show different nutrient content value: But when it comes to...
18 July 2024 9,907 12 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,669 5 View