I dont really understand what are you asking? What ever data you obtain from your experiments probably deserves to be published! It wont become new anticancer drug/s but its def. result as any other! It important what else except MTT assay you have done to show other mechanism of action that will support your thesis/assumptions...
I dont really understand what are you asking? What ever data you obtain from your experiments probably deserves to be published! It wont become new anticancer drug/s but its def. result as any other! It important what else except MTT assay you have done to show other mechanism of action that will support your thesis/assumptions...
I am getting ic 50 value of 300 microM and 1 milliM for two phytochemical drugs on doing MTT assay.People are objecting that i cant proceed with such a high value of ic 50 for further experiment to show some effect of drug on cell signaling pathways.
Well it depends on your funds and equipment and things that you have and that you can work freely with them.... :)
It depends on your aim, but basically, you should try to minimize the concentration as much as possible.
All (phytochemical) drugs will become toxic with high concentration. Thus, if cell death is your aim (e.g for anticancer drug discovery), you should never work with concentrations exceeding 50 µM (though already quite high). Working with high concentrations means that your compounds have a poor efficacy.
A major goal in drug development is to obtain compounds that are active at a nanomolar level.
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