Hello Navneet! I would suggest Low meting point agarose since it is the most used. It is a good way to avoid high temperatures on your preparation. A secondary choice would be to fix the tissue simply by using dissection pins. In order to do this you will need a recording chamber with a soft bottom i.e filled whit silgard.

Hope this works for you, regards!

Thanks Gaspar and Philipp for your valuable replies. I did try low-melting agarose at 4% but without any success.

It was heartening to see that the agarose and the tissue held themselves together while being sectioned. However the tissue wasn't attached to the agarose at the end of the sectioning cycle. I tried various speeds and vibrating frequencies for this.

I will try the agitation system and see if it makes a difference before I write to you.

Thanks again!