I've been trying to get Cre-recombinase immunohistochemistry to work on mouse sections of a Cre-expressing line.

I've tried two different Cre antibodies reported to work in other publications. I've tried different concentrations and different serums.

However neither of them works and I've got no clue of how to get it working.

My protocol is:

3x 5 mins PBS washes

2 hours in PBST (0.1%) with 5% Serum

Overnight at 4degrees in antibody in PBST with serum

3x 5 mins PBS washes

2 hours at RT in secondary antibody in PBST with serum

DAPI and PBS washes

Mounting

Any help?

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