Navneet Vasistha

5 Questions 21 Answers 0 Followers

Questions related from Navneet Vasistha

Good antibody for Beta IV Spectrin protein?

Hey guys, I'm looking for an antibody against Beta IV Spectrin to be used in both mouse and human sections. I'd like to use it for performing immunohistochemistry on sections. If you've used a...

08 August 2016 5,493 8 View

What statistical analysis could I do for a Sholl analysis experiment?

I'm comparing the arborisation of oligodendrocytes using the Fiji Sholl plugin and have 7 different conditions (cell lines) and variable number of cells counted for each line. Since no two cells...

07 July 2015 412 4 View

What materials other than agarose could I use for embedding unfixed tissue for electrophysiology?

I need the tissue to be healthy for ephys studies and so any processing should be under an hour and preferably around room temperature.

06 June 2014 4,168 2 View

Does anyone have a transfection/lipofection protocol for neural rosettes?

I would like to deliver some reporter plasmids into neural rosettes without lifting them from the coating. I was wondering if anyone has a protocol for doing so either by viruses or by lipofection...

04 April 2014 821 3 View

Cre immuno doesn't work on free-floating sections?

I've been trying to get Cre-recombinase immunohistochemistry to work on mouse sections of a Cre-expressing line. I've tried two different Cre antibodies reported to work in other publications....

04 April 2013 2,615 23 View