Hi Saber,

There are a few different methods you can use for inducing gastric cancer. If you are purely interested in looking at gastric cancer development regardless of your mouse genetic status I would recommend infecting with Helicobacter. You could either use H. pylori-SS1 which is a mouse adapted strain or H. felis which is derived from cats but infects mice very well. There are pros and cons when using either strain.

If you are unable to infect then you could use a carcinogen. Adding N-methyl-N-nitrosourea (MNU) to drinkning water has been shown to effectively cause gastric tumours (yamachika etal 1998).

Alternatively, you could use a genetically modified model of gastric cancer. There are quite a few excellent models which include inflammatory based models such as the gp130757FF mutant, Keratin-19/IL-1beta transgenic and COX2 overexpression or models which disrupt a regulatory function of the gastric mucosa and include gastrin null mice, H+K+ ATPase subunit knockouts, deficiencies in ion transporters, and TFF1-/- and TFF2-/- mice.

I hope this information is helpful.

perfect very very thank you