I have induced colon cancer by DMH in male rat with 180 gr weight .
I have injected DMH twice a week for 2 weeks and then after 4 weeks I have fixed the colon tissue in formalin 10% for 24h , then put it in paper filter and stained it with methylene Blue 0.2% for 20 min . (without sectioning and with a light microscope 40X )
Please help me for identifying the ACFs , here are the photos of my tissue samples .