What markers are best used to distinguish M1 and M2 macrophages in IHC?

More Irene Tan's questions See All

What is the principle/mechanism behind aging of carbon (graphite) containing refractory mix for isostatically pressed refractories?

The resin bonded carbon containing refractories are aged before use. How time of aging is determined? And mechanism behind aging.

23 July 2024 3,205 0 View

When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...

21 July 2024 7,284 4 View

How to display grain boundaries in RVE model?

Many literatures have shown that the RVE model shows the grain boundaries between different grains. How can this be achieved using DAMASK+paraview?

21 July 2024 9,224 1 View

Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...

19 July 2024 148 6 View

What is the future scope of acoustic emission?

17 July 2024 1,510 1 View

Is the protecting group boc of the amino group stable at 37°C?

I have a small molecule reagent with a boc-protected amino group. Now the reaction needs to be reacted at 37°C for 30 h. Is this protection group stable?

12 July 2024 3,745 2 View

Why can't I detect the plasmon resonance angle with water?

I am trying to measure the plasmon resonance angle of gold film and pure water using the Kretschmann configuration and a 633nm laser. Without flowing water over the gold, I can detect a clear...

10 July 2024 4,719 3 View

Gaussia Luciferase for Bioluminescence Tumor Monitoring in Comparison with Firefly Luciferase. Which is better?

Been doing searches to find the best paper to start understanding the advantages and disadvantages of each technology: Gaussia Luciferase for Bioluminescence Tumor Monitoring in Comparison with...

29 June 2024 9,856 1 View

Is it better to use 2 intact classes for the quasi-experimental study?

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25 June 2024 6,244 3 View

How can we generate topology file for water and helium system?

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24 June 2024 1,242 0 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

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06 August 2024 666 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

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How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

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What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...

01 August 2024 476 2 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

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Culture fibroblasts with or without FGF2?

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While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

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29 July 2024 2,682 6 View

Human macrophages cell surface markers?

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Michael Förster

Hi Irene Tan

Check out the following paper for M1/M2 macrophage polarization and specific markers.

Article Novel Markers to Delineate Murine M1 and M2 Macrophages

In the context of parasitic infection and pending on the mouse strain used in your experiment, there may be value checking out Ym1/ Chi3l3 as per:

Article Natural polymorphism of Ym1 regulates pneumonitis through al...

All the best & good luck with your experiments,

Michael

Irene Tan

Hi Michael Förster, thanks a lot. I will go over these papers.