What marker (other than CD14) should I use to screen monocyte derived macrophages from monocytes and dendritic cells?

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Root-AMF colonization analysis non-destructive method?

Is there any non-destructive method (not to harvest the root) to quantify AMF colonization with plant roots in pot experiments or rhizotrons?

02 October 2023 9,449 0 View

I am working on graphene based supercapacitor I wanted to know how to make a comparison between the estimated capacitance of CV and GCD?

Please suggest me as they differ in their measurement principle

11 April 2023 2,775 1 View

Can anyone suggest where would I get the codes(C++ or any) for charge exchange cross-section of positive to negative ion converting atomic reactions?

The codes to plot the cross-section vs energy curve and also the research article that might help in understanding, theoretically and mathematically, the reactions of the form: A^+ + B^-...

15 January 2023 8,925 2 View

How do you determine what the compound actually is based on it's m/z ratio?

I did a metabolomic study, where some of my compounds are novel and have high production in one of my mutants. I have its m/z ratio. Can I identify this compound solely based on this ratio? I...

06 October 2022 8,248 4 View

What is the basic difference between time-correlated single photon counting (TCSPC) and Fluorescence Lifetime Imaging microscopy (FLIM)?

Is there any difference between time-correlated single photon counting (TCSPC) and Fluorescence Lifetime Imaging microscopy (FLIM)? TCSPC i.e. lifetime measurement of the fluorophore is basically...

01 September 2022 5,780 6 View

Do I have to do RNAcleanup after DNAseI treatment and heat inactivation of DNAse?

I am extracting RNAs using Trizol combined with Purelink mini kit and I am doing post isolation DNAse treatment for making RNA free from genomic DNA. After DNAse treatment I do inactivate DNAse...

02 November 2021 7,086 2 View

Hi there! Do anyone has experience in terms of efficiency of on column DNAse treatment vs post extraction DNAse treatment during RNA extraction?

I am doing RNA extraction from pea plant roots for sequencing purpose and require good quality RNA without contamination. I am using Purelink RNA Mini Kit for extraction. I found on column DNAse...

28 October 2021 2,344 0 View

How to plot force vs time plot and force-extension plot from Steered MD Simulation data?

I have done steered MD of RNA and now trying to figure out the analysis of the data obtained by the simulation.. I wanted to know on how to plot the force vs time plot and force-extension plot...

26 October 2021 4,964 2 View

Any experimental data on the stiffness properties of interface in debris to rockmass contact?

Fellow researchers, please help me in getting some experimental results which I can use for determining the interface stiffnesses (normal and shear), cohesion and friction angle values for debris...

16 July 2021 4,292 5 View

How to make index file in gromacs for nitrogen bases only?

I am working on GROMACS software and I wanted to know, how can select only nitrogen bases for making index file in GROMACS.. I am stuck.. Can anyone please help me.. Is there any script or command...

10 July 2021 8,315 2 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

What should Berlin do as a city to become as impactful as London and Paris in World Football?

Please go through my Abstract. I can also share a proposed Thesis Outline.

04 August 2024 2,077 0 View