It may be related to its protein structure or enzyme activity. At different temperatures, the virus's shell or enzyme activity may change.

Thanks, that what I thought too but I wonder if someone know what happen more precisely. Which protein will change and what will be the inhibited function then. The firsts papers talking about phae159 only mention the temperature sensitive feature of it with no more details. Maybe nobody knows exactly what happen.

Dear Dr. Flavien Maucourt

From what I could understand from the two articles attached below is that thermosensitive mutations are created in the engineered mycobacteriophage phAE159 genome that allows replication at 30 deg C but not at 37 deg C.

References:

Article Specialized Transduction: an Efficient Method for Generating...

Article Cluster K Mycobacteriophages: Insights into the Evolutionary...

Regards,

Malcolm Nobre

Thank you for your answer

Prob based on protein tertiary structure.

True ! Thanks