Hello,

I have a technical question for you.

For RNA and DNA extraction, we use Quiagen kit with Trizol/thiocyanate de guanidine lysis.

However, it's a bit long and we wanted to improve our methods.

Promega propose to us to use their kit "Maxwell plus RSC FFPE kit" that have a great yield for FFPE tissus.

Here, we work on frozen tissus and we lyse them with Trizol/thiocyanate de guanidine and mechanical grinding.

The lysis of Promega is long, under ambient temperature and we think it will degrade nuclic acid after extraction.

We wanted to know if a lab had the same issues ? Have you a lysis advice to choose ?

Thank you by advance,

Claire