Bharatendu Singh

2 Questions 3 Answers 0 Followers

Questions related from Bharatendu Singh

I want to do a site directed mutagenesis to change the amino acid sequence in the expressed product. I have some ques. regarding primer design?

While designing the mutagenic primers for substitution, my final primer sequences have two mismatches with the template for the necessary mutation to be present in the final sequence. For e.g.,...

21 October 2021 9,731 4 View

Currently i am culturing Influenza A in MDCK cell line and i wanted to ask is it possible to obtain high titre of virus without adding TPCK trypsin?

I have done 3 blind passages to concentrate the viral stock in the supernatant without adding TPCK Trypsin. After passage 1 my Ct value for INF A was 36.96. After P2 the value decreased to 33.36....

22 October 2020 4,087 3 View