Thanks !
Rodents will receive calcium injection to specify preferred tissue distribution after my drug treatment. Measuring radioactivity from blood sample is fairly easy, is it feasible to determine...
09 October 2018 7,637 5 View
I will have bone tissue embedded on the microscope slide/parafin AND vascular tissue where chondrocytic cells/bone cells might be present as a atherosclerotic plaque. I was wondering whether...
10 November 2017 9,200 4 View
Is there such an existing kit or maybe electrophysiology experiment ?
06 July 2017 5,219 2 View
Thanks!
02 March 2017 5,288 0 View
I have transfected HEK293 cells with NR1/2 subunits. I am currently using 1mM of NMDA in the activation buffer. Is this value too high/too low? Thanks
04 May 2016 381 5 View
I am trying to detect proteins using Western Blot Technique. Due to the size difference, smaller protein of 36kDa was not seen on the blot read-out. Would gradient gel be a better idea to detect...
31 December 2015 4,280 10 View
If so what did you do in terms of assays?
01 January 1970 6,021 0 View
Could you recommend some articles on Urban Transportation System optimization and Innovation?
13 August 2024 2,595 3 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
