If so what did you do in terms of assays?
Rodents will receive calcium injection to specify preferred tissue distribution after my drug treatment. Measuring radioactivity from blood sample is fairly easy, is it feasible to determine...
09 October 2018 7,637 5 View
I will have bone tissue embedded on the microscope slide/parafin AND vascular tissue where chondrocytic cells/bone cells might be present as a atherosclerotic plaque. I was wondering whether...
10 November 2017 9,200 4 View
Is there such an existing kit or maybe electrophysiology experiment ?
06 July 2017 5,219 2 View
Thanks !
02 March 2017 1,680 0 View
Thanks!
02 March 2017 5,288 0 View
I have transfected HEK293 cells with NR1/2 subunits. I am currently using 1mM of NMDA in the activation buffer. Is this value too high/too low? Thanks
04 May 2016 381 5 View
I am trying to detect proteins using Western Blot Technique. Due to the size difference, smaller protein of 36kDa was not seen on the blot read-out. Would gradient gel be a better idea to detect...
31 December 2015 4,280 10 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...
03 August 2024 835 3 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
