What is the best way to detect de novo calcium accumulation after i.v. injection of calcium (CaCl2 + Ca45 as tracer) in rodent organs?

Rodents will receive calcium injection to specify preferred tissue distribution after my drug treatment. Measuring radioactivity from blood sample is fairly easy, is it feasible to determine...

09 October 2018 7,481 5 View

Which staining would you recommend to stain chondrocytic cells (connective tissue, collagen?) bone cells (osteoblast, osteocasts, osteocytes) ?

I will have bone tissue embedded on the microscope slide/parafin AND vascular tissue where chondrocytic cells/bone cells might be present as a atherosclerotic plaque. I was wondering whether...

10 November 2017 9,134 4 View

Can anyone recommend any reliable methods of measuring the ATP levels in cardiac myocytes (cell line or primary cell line)

Is there such an existing kit or maybe electrophysiology experiment ?

06 July 2017 5,120 2 View

What live/dead cell assay would you recommend for cells encapsulated in alginate gel system?

02 March 2017 1,604 0 View

How many smooth muscle cells should I seed per well in 48-well plate along with alginate gell encapsulation?

Thanks!

02 March 2017 5,237 0 View

Whats the optimum concentration of NMDA or glutamate to activate NMDA receptor expressed in HEK293 cells ?

04 May 2016 314 5 View

What is the optimum percentage of SDS-PAGE for proteins of mass 34kDa and 175kDa when both need to be loaded on one gel ?

I am trying to detect proteins using Western Blot Technique. Due to the size difference, smaller protein of 36kDa was not seen on the blot read-out. Would gradient gel be a better idea to detect...

31 December 2015 4,194 10 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View