Grzegorz Wasilewski

8 Questions 7 Answers 0 Followers

Questions related from Grzegorz Wasilewski

What is the best way to detect de novo calcium accumulation after i.v. injection of calcium (CaCl2 + Ca45 as tracer) in rodent organs?

Rodents will receive calcium injection to specify preferred tissue distribution after my drug treatment. Measuring radioactivity from blood sample is fairly easy, is it feasible to determine...

10 October 2018 7,602 5 View

Which staining would you recommend to stain chondrocytic cells (connective tissue, collagen?) bone cells (osteoblast, osteocasts, osteocytes) ?

I will have bone tissue embedded on the microscope slide/parafin AND vascular tissue where chondrocytic cells/bone cells might be present as a atherosclerotic plaque. I was wondering whether...

11 November 2017 9,183 4 View

Can anyone recommend any reliable methods of measuring the ATP levels in cardiac myocytes (cell line or primary cell line)

Is there such an existing kit or maybe electrophysiology experiment ?

07 July 2017 5,200 2 View

How many smooth muscle cells should I seed per well in 48-well plate along with alginate gell encapsulation?

Thanks!

03 March 2017 5,272 0 View

What live/dead cell assay would you recommend for cells encapsulated in alginate gel system?

Thanks !

03 March 2017 1,656 0 View

Whats the optimum concentration of NMDA or glutamate to activate NMDA receptor expressed in HEK293 cells ?

I have transfected HEK293 cells with NR1/2 subunits. I am currently using 1mM of NMDA in the activation buffer. Is this value too high/too low? Thanks

05 May 2016 360 5 View

What is the optimum percentage of SDS-PAGE for proteins of mass 34kDa and 175kDa when both need to be loaded on one gel ?

I am trying to detect proteins using Western Blot Technique. Due to the size difference, smaller protein of 36kDa was not seen on the blot read-out. Would gradient gel be a better idea to detect...

01 January 2016 4,252 10 View

Has anyone grown VSMC's (vascular smooth muscle cells) in alginate?

If so what did you do in terms of assays?

01 January 1970 6,006 0 View