I am specifically interested in synthesizing cyclotides with a small peptide graft as described in the experimental procedures of the paper: (Rasmus Eliasen et al. "Design, Synthesis, Structural and Functional Characterization of Novel Melanocortin Agonists Based on the Cyclotide Kalata-B1"). SPPS is the de facto methodology for production of these proteins. I have a $1000 budget for this procedure. Does this process require a high amount of laboratory training and/or cost compared to E. Coli expression of a more standard protein type?
SPSS can be fairly expensive if you have to buy everything for the first time. It's usually cheaper to order the peptides as a custom synthesis http://www.genscript.com/peptide.html
The main problem with your peptides is that you have multiple disulfide bonds which may be difficult
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