Hi,

First you can check your primer design, and be sure that your primers target only your gene, and also you can change your annealing temperature, increasing annealing temperature can eliminate pseudo gene amplification.

Yes, there is a miss hit in annealing which is getting you off product. Try to make primers with higher GC corners and with higher annealing temperature.

Hi, thanks for suggestions. I will try increase annealing T first and see how it works.