What kind of experiments can be conducted for testing resistance dual gene transgenic peanut plants?

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How to estimate sample size for GWAS of continuous and discrete traits? What are the pre-requisites?

Genome-wide association study (GWAS) Continuous traits: eg. Height Discrete traits: eg. Eye color

28 July 2024 286 0 View

Can we fit a gaussian Fit on a LIBS Peak ?

Though much literature suggests fitting a Voigt profile on a LIBS peak, is it also possible to fit a Gaussian profile on the LIBS peak? I have 204 spectra files, and I am building a pipeline for...

23 July 2024 6,127 1 View

Why is my phage library obtained at round 2 of Biopanning viscous?

After incubation of the TG1 cells with the helper phage, I spun down the culture and precipitated the supernatant with PEG/NaCl. After this, I spun it down again and resupended the pellet in PBS....

21 June 2024 6,260 2 View

Can we use LIBS Peak - Ti 441.492 for the Univarate Analysis ?

I have 17 different steel samples, in which i identified a peak of Ti 441.492. But , if you look closely at photo, while some some peak are at 441.492 , some are at the slight offset. eg 441.490...

10 June 2024 8,934 2 View

Challenges in Univariate Analysis for Stainless Steel Samples using Laser Induced Breakdown Spectroscopy?

Respected Members , I have a 17 different steel sample references , with varying concentration for Minor Elements being Mo , Ni , Mn ,Si , Cr etc , i am trying to build a calibration curve with...

10 June 2024 993 0 View

Normalization in LIBS Spectra ?

Hello Everyone ! I have a samples a satinless steel , I performed the LIBS Measurement with hLIBS (SciAps Z-300). Total of 12 measurements on different locations were done on the same sample...

09 May 2024 7,959 6 View

Is IJSREM UGC Care or cloned journal?

Some journals seems to be fake..cloned

07 May 2024 3,623 1 View

Does anyone know about Universities offering (a) PhD by prior publication or (b) PhD by portfolio?

Dear Colleagues, Does anyone know about Universities that are offering (a) Ph.D. by prior publication (b) Ph.D. by portfolio? I have two publications viz."Regression Testing in Era of Internet of...

09 April 2024 2,123 1 View

How to calculate chemical quantity required when relative molar composition is given ?

Relative molar composition of 1.0 TEOS : 0.2 CTACl : 0.0026–0.017 LS277 dye : 10.4 TEA : 142 H2O was used.

04 April 2024 6,770 1 View

What are usual pumping and heating costs in the refineries?

As heat exchangers in the heat exchanger networks get fouled, the utility in the furnace has to increased to compensate the losses. Apart from it, the pumping cost must be increasing as deposited...

04 February 2024 531 3 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

How can I find access to heavy metal reference doses from the EPA and WHO websites?

Reference dose and Maximum acceptable concentrations HMs

03 August 2024 8,230 4 View

What is the best sampling strategy?

I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...

01 August 2024 8,457 10 View

Technology developments may it for whatever reasons developed eventually its benefits are multi-Disciplinatary?

Your comments

28 July 2024 2,822 2 View

New and trending areas in the field of production technology using microorganisms?

Novel areas especially

24 July 2024 8,416 0 View

I encountered a situation where the 16s PCR amplification worked perfectly with the DNA from the same sample, but using the same sample Don't working?

I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...

24 July 2024 9,688 0 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

Seeking latest research on social protection in northeast Nigeria, Yobe state. How does it compare with other states?

What has been the social protection outreach like in Yobe state. Which areas are challenging for the government departments and what capacity building would be most effective? Comparison of the...

21 July 2024 9,887 1 View

Bhupendra singh Punwar

Hi abhinandan,

First perform Southern followed by qPCR and then Wastern or DAC ELISA (If you have antibody against your transgene or if you have tagged transgene)

Abhinandan Surgonda Patil

Thanx.........we don't have currently antibodies

This was combined Project of DGR and Advanced centre of plant virology (IARI)

The Co-Pi was DR R K Jain But he get promoted we dont know now to whom to contact....

Sir can you give the details of any company that can provide the antibody to us.